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KMID : 0380319900460000169
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Journal of Korean Research Institute for Better Living
1990 Volume.46 No. 0 p.169 ~ p.176
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Identification of Intermediate of the Metabolism of Methylthioadenosine by Cell-free Extracts of Actinoplanes ferrugineus
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Abstract
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To identify intermediate(s) during the conversion of methylthioadenosine to methionine by cell-free extracts of Actinoplanes ferrugineus, a compound was isolated from the anaerobic incubation mixture of cell-free extracts and CH_3-[^14C]-methylthioadenosine, followed by 0.01 N―HCI hydrolysis. The compound was characterized through alkaline phosphatase treatment, NaBH_4 reduction, and NaBH_4 reduction―periodate oxidation reaction. The intermediate seemed to be 1-phospho-5-methylthio-5-deoxyribulose.
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KEYWORD
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